Anti-tdTomato

Catalog #
AB8181
Family
Fluorescent Proteins
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF, ELISA
Reactivity
Transfected cells
Description
Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.
Alternative names
Cherry fluorescent protein; DsRed, mCherry, red fluorescent protein, RFP antibody.
Immunogen
Purified recombinant peptide produced in E. coli.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Form
​Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500
ELISA ND

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ +++ +++ +++ ND

Images

Immunofluorescence – anti-tdTomato Ab in 293HEK cells transfected with cDNA at 1/50 dilution; cells were fixed with 4% of PFA;
Anti-tdTomato Ab at 1/1,000 dilution; 293HEK cells transfected with cDNA; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

600 µg
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