Anti-GFP, DyLight®550

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Catalog #: AB66550
Family: Fluorescent Proteins
Category: Antibodies
Source: Goat
Application: WB, IHC (F), IF
Reactivity: Transfected cells
Description: Goat polyclonal antibody to GFP (green fluorescent protein) conjugated to DyLight® 550. GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Alternative names: Green fluorescent protein antibody.
Immunogen: Purified recombinant peptide produced in E. coli.
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form: Polyclonal antibody supplied as a 100 (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide.
Concentration: 2 mg/ml
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
Special instructions: Avoid freeze/thaw cycles.

Usage

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Transfected cells	+++	+++	ND	+++	ND

Images

	Immunofluorescence –anti-GFP Ab conjugated to DyLight® 550 using hCEC cells transduced with GFP-Rab1a; cells were fixed with methanol and anti-GFP at 1/250;
	Immunofluorescence in Drosophila larvae NMJ syt1-GFP (syt1 is a synaptic protein) muscle 6/7 using anti-GFP conjugated to DyLight®550 at 1/500;
	Anti-GFP Ab conjugated to DyLight® 550 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane;
	Immunofluorescence in Drosophila larvae NMJ syt1-GFP (syt1 is a synaptic protein) muscle 6/7 using anti-GFP conjugated to DyLight®550 at 1/500;