Anti-GFP, DyLight®488

Catalog #
AB66488
Family
Fluorescent Proteins
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to GFP (green fluorescent protein) conjugated to DyLight® 488. GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Alternative names
Green fluorescent protein antibody.
Immunogen
Purified recombinant peptide produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 100 (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide.
Concentration
2 mg/ml
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:1,000
IF 1:50-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ +++ ND +++ ND

Images

Anti-GFP Ab conjugated to DyLight® 488 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane;
Immunofluorescence – anti-GFP Ab conjugated to DyLight® 488 using hCEC cells transduced with GFP-Rab1a (signal amplification); cells were fixed with methanol and anti-GFP at 1/250;
Immunofluorescence – anti-GFP Ab conjugated to DyLight® 488 using hCEC cells transduced with GFP-Rab1a (signal amplification); cells were fixed with methanol and anti-GFP at 1/250;

350 €

Quantity / Unit Size

250 µg
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