Anti-GFP, DyLight®405
- Catalog #
- AB66405
- Family
- Fluorescent Proteins
- Category
- Antibodies
- Source
- Goat
- Application
- WB, IHC (F), IF
- Reactivity
- Transfected cells
- Description
- Goat polyclonal antibody to GFP (green fluorescent protein) conjugated to DyLight® 405. GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
- Alternative names
- Green fluorescent protein antibody.
- Immunogen
- Purified recombinant peptide produced in E. coli.
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Form
- Polyclonal antibody supplied as a 100 (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide.
- Concentration
- 2 mg/ml
- Specificity
- In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
- Special instructions
- Avoid freeze/thaw cycles.
-
Application Values WB 1:500-1:5,000 IHC (F) 1:50-1:1,000 IF 1:50-1:1,000 -
Sample WB IHC (F) IHC (P) IF ELISA Transfected cells +++ +++ ND +++ ND -
Immunofluorescence –anti-GFP Ab conjugated to DyLight® 405using COS-7 cells transduced with GFP-Rab5a; cells were fixed with methanol and anti-GFP at 1/250;
Usage
Reactivity Chart
Images
350 €
Quantity / Unit Size
250 µg
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