Anti-Goat IgG, DyLight®550

Catalog #
AB200550
Family
Secondary Antibodies
Category
Antibodies
Source
Chicken
Application
WB, IF
Reactivity
Goat
Description
Chicken polyclonal antibody to goat IgG conjugated to DyLight®550.
Alternative names
Immunoglobulin Y antibody.
Immunogen
Full goat IgG affinity purified from domestic goat (Capra hircus) serum using protein G. Purity >95% by SDS-PAGE.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 200 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration
2 mg/ml
Specificity
Using serum samples detects goat IgG by Western blot. Reduced cross-reactivity to other species.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:2,000-1:10,000
IF 1:100-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Goat +++ ND ND +++ ND ND

Images

Anti-GAPDH Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; chicken polyclonal to goat IgG conjugated to DyLight® 550 (AB200550) at 1/10,000 dilution;
Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing GluRIIA-GFP in neurons (GluRIIA is a post-synaptic protein) using 1st Ab anti-GFP at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight®550 at 1/500;

200 €

Quantity / Unit Size

500 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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