Anti-beta-Actin

Catalog #
AB0145
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Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Actin proteins are highly conserved and ubiquitously expressed in all eukaryotic cells. These proteins are the major components of the cytoskeleton and involved in various types of cell motility.
Alternative names
ACTB, beta-actin, BRWS1, PS1TP5BP1 antibody.
Immunogen
Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli.
Storage
For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:250-1:2,000
IF 1:50-1:250
IHC (F) 1:250-1:1,000
IHC (P) 1:250-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ +++ ND
Rat +++ +++ +++ +++ ND
Mouse +++ +++ +++ +++ ND
Canine +++ +++ +++ +++ ND
Monkey +++ +++ +++ +++ ND

Images

Anti-beta-Actin Ab at 1/500 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
IHC of mouse kidney using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.
Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin;
IHC of mouse liver using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.
Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin;
IHC of human lymph node using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.
Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin;
IHC of human appendix using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.
IHC of human mouse heart using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.

320 €

Quantity / Unit Size

600 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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