Anti-CD31

Catalog #
AB0092
Family
CD Markers
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P)
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to CD31. CD31 is a 130-140 kDa type I transmembrane glycoprotein. It is expressed in platelets, granulocytes, stem cells of myeloid lineage, monocytes and endothelial cells. It is likely to be involved in integrin activation, leukocyte migration and angiogenesis.
Alternative names
CD31/endoCAM, endoCAM, PECA1, PECAM1, PECAM-1, platelet/endothelial adhesion molecule 1 antibody.
Immunogen
Purified recombinant peptide within residues 690 aa to the C-terminus of human CD31 produced in E. coli.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
Using PBM cell lysates detects a 130-140 kDa band by Western blot.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
IHC (F) 1:250-1:1,000
IHC (P) 1:250-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ ND ND
Rat +++ +++ +++ ND ND
Mouse +++ +++ +++ ND ND
Canine +++ +++ +++ ND ND
Monkey +++ +++ +++ ND ND

Images

IHC of human lymph node using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.
Endogenous CD31 detected at 1/500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
IHC of human breast carcinoma using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.
IHC of mouse spleen using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.
IHC of mouse lung using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.
IHC of mouse kidney using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.

300 €

Quantity / Unit Size

600 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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