Anti-GAPDH

Catalog #
AB0049
Family
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Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Human, Rat, Mouse, Monkey, Zebrafish, Canine
Description
Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Alternative names
glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
Immunogen
Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Form
Polyclonal antibody supplied as a 200 or 500 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2 mg/ml
Specificity
Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Special instructions
The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IF 1:50-1:250
IHC (P) 1:200-1:1,000
IHC (F) 1:200-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ +++ ND
Rat +++ +++ +++ +++ ND
Mouse +++ +++ +++ +++ ND
Zebrafish +++ ND ND ND ND
Canine +++ +++ +++ +++ ND
Monkey +++ +++ +++ +++ ND

Images

Anti-GAPDH Ab at 1/2,500 dilution; lysates at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Anti-GAPDH Ab at 1/2,500 dilution; lysates at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-GAPDH Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with methanol;

320 €

Quantity / Unit Size

400 µg
1 mg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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